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4-HNE forms stable covalent adducts to proteins, which can be detected by immunostaining with specific antibodies 15,16
Balance matters
The following reagents were also used in our study: 1640 (Gibco, Grand Island, United States), FBS (Gibco, Grand Island, United States), trypsin (Amresco, United States), EDTA (Amresco, United States), ELISA Kit for TNF- and IL-6 (R&D Systems, Minneapolis, United States), Annexin V-FITC/PI Kit (BD, San Jose, United States), APC-conjugated anti-mouse CD8 (BioLegend, San Diego, United States), BV421-conjugated anti-mouse CD86 (BioLegend, San Diego, United States), BV711-conjugated anti-mouse CD64 (BioLegend, San Diego, United States), FITC anti-mouse MERTK Monoclonal Antibody (BioLegend, San Diego, United States), PECY7-conjugated anti-mouse CD11b (BioLegend, San Diego, United States), PE-conjugated anti-mouse CD3 (BioLegend, San Diego, United States), PEconjugated anti-mouse B220 (BioLegend, San Diego, United States), AlexaFluor 594 anti-mouse CD326 (Ep-CAM) Antibody (BioLegend, San Diego, United States), Alexa Fluor 647 mouse anti-Siglec-F (BioLegend, San Diego, United States), eFluor 570 mouse anti-F4/80 (BioLegend, San Diego, United States), eBioscience Fixable Viability Dye eFluor 780 (eBioscience, San Diego, United States), DNase I (Roche, Basel, Switzerland), DTT (MerckRoche, Basel, Switzerland), collagenase IV (Sigma-Aldrich, St
